Which deficiency is most likely responsible for impairing the respiratory burst in phagocytes?

• A. Myeloperoxidase
• B. Catalase
• C. NADPH oxidase
• D. Superoxide dismutase

Answer: (C)

The respiratory burst in phagocytes is primarily characterized by the rapid production of reactive oxygen species (ROS) used to destroy engulfed pathogens. This process is heavily dependent on the function of NADPH oxidase, an enzyme complex that catalyzes the reduction of oxygen to superoxide, a key component in the generation of ROS during the respiratory burst.

A deficiency in NADPH oxidase directly impairs the ability of phagocytes, such as neutrophils and macrophages, to produce superoxide and subsequently other reactive species involved in microbial killing. This can lead to a condition known as Chronic Granulomatous Disease (CGD), where individuals are prone to recurrent bacterial and fungal infections due to the inability of their immune cells to effectively respond to pathogens.

The other enzymes listed contribute to the breakdown of ROS or assist in the process but do not initiate the respiratory burst. For example, myeloperoxidase works downstream of NADPH oxidase and utilizes hydrogen peroxide to form hypochlorous acid, while superoxide dismutase and catalase serve protective roles against oxidative stress rather than driving the initial oxidative burst. Therefore, a deficiency in NADPH oxidase is the most significant determinant affecting the respiratory burst in phagocytes.